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Advanced course on biotesting - Its application in environmental health and ecotoxicology

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Time: November 13 - December 4, 2006

Place: University of Kuopio, Kuopio (Maps and connections)

Medistudia (the building number 3 on the map)

Canthia (the building number 1 on the map)

Bioteknia (the building number 14 on the map)

Tietoteknia (the building number 13 on the map)

Credits: 3.0 credit points

Language: English

Organizer: Environmental Risk Assessment Centre (ERAC)

Course leader: Atte von Wright, Professor, University of Kuopio, Kuopio

Course description: The course includes a short introduction to basics of cell culture techniques, microbiology and genetics, followed by a detailed description of short term toxicity testing and introduces some new approaches for environmental risk assessment. Learning task gives an opportunity to critically evaluate and apply environmental risk assessment in practise.


Lecturers:

Ulla Honkalampi-Hämäläinen (UHH) University of Kuopio, Dept of Applied Biotechnology, Kuopio

Dr Jani Honkanen (JH) University of Joensuu, Laboratory of Aquatic Ecology and Ecotoxicology, Joensuu

Professor Jussi Kukkonen (JK) University of Joensuu, Laboratory of Aquatic Ecology and Ecotoxicology, Joensuu

Professor Matti Leppänen (ML) University of Joensuu, Laboratory of Aquatic Ecology and Ecotoxicology, Joensuu

Dr. Annalaura Stammati (AS) Istituto Superiore di Sanità (Italian National Institute of Health) Rome, Italy

Professor Atte von Wright (AvW) University of Kuopio, Dept of Applied Biotechnology, Kuopio


Further information: Sari.Makkonen(at)uku.fi, Tel. +358-17-163188


Time schedule:


DateLecture
November 13
Medistudia, MS1+2
9.15-11.00Basics of cell culture techniques UHH
12.15-14.00Basics of microbiology AvW
14.15-16.00Basics of genetics AvW
November 15
Canthia, S12
8.15-10.00Acute cytotoxicity tests UHH

- TPC

- HTD

10.15-12.00Sublethal cytotoxicity tests UHH

- RNA synthesis inhibition

- spermatozoan motility inhibition

- others

13.15-15.00Genotoxicity tests AvW

- microbiological tests

- in vitro tests (cell culture)

- in vivo tests

November 16
Bioteknia 2, Lab. 2409, 2412
10.15-16.00Distribution of group works

Demonstrations:

- BSM (boar sperm motility inhibition assay) UHH

- HTD (morphological changes, highest tolerated dose) UHH

- AMES -test AvW

November 17
Tietoteknia, 1038
9.15-11.00Aquatic toxicity tests JH
12.15-14.00Ecological testing of contaminated sediments ML
14.15-16.00Biomarkers and in situ testing JK
December 4
Canthia, S12
10.15-12.00Process validation of biotests AS
13.15-15.00Seminar: Presentation of the results of group works

Kurssimateriaali:





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